BioSpectrometer Fluorescence
 
The Eppendorf BioSpectrometers are spectrophotometers that combine easy handling with highly reproducible analysis. un variety of cuvettes expands the opzioni even further from microvolume a macrovolume measurements.
 
The Eppendorf BioSpectrometer fluorescence is an expansion of the Eppendorf BioSpectrometer basic and features an integrated fluorescence module. Measurements can be flexibly executed in the UV/Vis or fluorescence range. The Eppendorf BioSpectrometer fluorescence can be used a increase the measuring range for biomolecules (nucleic acids and proteins) by un factor of 1,000, compared a UV measurements. What's more, fluorescence dyes allow un more accurate and reliable quantification due a the fact that these dyes bind specifically a the target biomolecule. UV/Vis spectroscopy and fluorescence are complementary methods. The Eppendorf BioSpectrometer fluorescence is the first spectrophotometer on the market which combines both measuring methods in one piccolo compact device. In combination with the microvolume cuvette, Eppendorf µCuvette® G1. 0, it can measure dsDNA from 1 pg/µL a 1,500 ng/µL.
 
Features
 
All Eppendorf BioSpectrometer basic opzioni
 
Preprogrammed and freely programmable fluorescence methods
 
Fluorescence measurement concentrazione of dsDNA: 1 pg/µL – 1,000 pg/µL
 
Fluorescence measurement concentrazione of BSA: 0. 01 ng/µL – 10 ng/µL
 
Fluorescence excitation wavelength 470 nm, emission wavelengths 520 nm and 560 nm
 
Fluorescence intensity across un range of 0. 5 nM a 2,000 nM fluorescein
 
Applications
 
dsDNA quantification down a 1 pg/µL with common kits, e. g. , Pico Green®
 
ssDNA quantification down a 10 pg/µL
 
RNA quantification down a 20 pg/µL
 
Oligo quantification down a 10 pg/µL
 
Protein (BSA) quantification down a 0. 01 ng/µL
 
Quantification of GFP-binding proteins
 
Growth measurement of GFP-binding bacteria
 
The Eppendorf BioSpectrometer Fluorescence Reference Filter Set: is used a verify photometric accuratezza wavelength systematic error, and fluorimetric precisione (random error) and linearity on the Eppendorf BioSpectrometer fluorescence.